Figure 1.—

Comparison of segregation patterns in strains with varying levels of DSB hotspot activity. The observed percentages of total aberrant segregation, gene conversion, and postmeiotic segregation events are indicated as Total Ab., GC, and PMS, respectively. As described in the results, we calculated restoration events (Rest.) by comparing the frequencies of aberrant segregation in wild-type and msh2 strains. (A) Strains with wild-type DSB frequency at the HIS4 hotspot. (B) Strains with wild-type DSB frequencies at the ARG4 and DED81 hotspots. (C) Strains homozygous for an insertion of telomeric sequence upstream of ARG4 (arg4-tel) that increases the DSB frequency at the ARG4 hotspot and decreases the DSB frequency at the DED81 hotspot. (D) Strains homozygous for the bas1 deletion, a deletion that eliminates the DSB located immediately upstream of HIS4. (E) Strains homozygous for the his4-51 mutation. This mutation eliminates the Rap1p-binding site upstream of HIS4 and eliminates the HIS4-associated DSB.