TABLE 2.
Diploid yeast strains
| Strain | Relevant homozygous mutations | Crossa |
|---|---|---|
| Strains heterozygous for his4-AAG | ||
| DTK224 | rad1∷ura3 | TP1011 × TP1010 (Kirkpatrick and Petes 1997) |
| HMY95 | mlh1∷kanMX4 | HMY105 × HMY91 (Welz-Voegele et al. 2002) |
| HMY96 | pms1∷kanMX4 | DNY95 × HMY92 (Welz-Voegele et al. 2002) |
| JSY136 | exo1∷kanMX4 | HMY118 × JSY133 |
| JSY163 | msh3∷kanMX4 | HMY110 × JSY143 |
| JSY164 | msh6∷kanMX4 | HMY134 × JSY144 |
| JSY165 | mlh2∷kanMX4 | HMY161 × JSY139 |
| JSY178 | mlh3∷kanMX4 | HMY158 × JSY137 |
| JSY291 | msh4∷kanMX4 | HMY223 × JSY151 |
| JSY297 | msh5∷hygB | JSY293 × JSY294 |
| JSY314 | pol3-01 | JSY307 × JSY312 |
| JSY320 | bas1∷hygB | JSY316 × JSY317 |
| JSY321 | bas1∷hygB msh2∷Tn10LUK7-7 | JSY318 × JSY319 |
| JSY338 | his4-51 | MW30 × JSY337 |
| JSY343 | his4-51 msh2∷kanMX4 | JSY336 × JSY342 |
| JSY349 | msh2∷kanMX4 msh4∷kanMX4 | JSY347 × JSY348 |
| No. 5 | msh2∷Tn10LUK7-7 | RJK1721 × RJK 1452 (Alani et al. 1994) |
| PD83 | Wild type | AS4 × PD73 (Detloff et al. 1991) |
| Strains heterozygous for his4-Sal | ||
| JSY188 | msh3∷hygB/kanMX4 | HMY110 × JSY184 |
| JSY210 | mlh2∷kanMX4 | HMY161 × JSY197 |
| JSY216 | mlh3∷kanMX4 | HMY158 × JSY211 |
| JSY217 | msh6∷kanMX4 | HMY134 × JSY193 |
| JSY230 | mlh1∷hygB/kanMX4 | HMY105 × JSY195 |
| JSY233 | pms1∷kanMX4 | HMY106 × JSY231 |
| JSY239 | rad27∷hisG | HMY123 × JSY267 |
| JSY240 | msh2∷kanMX4 | HMY104 × JSY206 |
| JSY255 | rad1:ura3 | DTK225 × JSY252 |
| JSY277 | mlh1∷hygB pms1∷kanMX4 | JSY274 × JSY273 |
| JSY283 | mlh1∷hygB mlh3∷kanMX4 | JSY149 × JSY261 |
| JSY284 | exo1∷kanMX4 | HMY118 × JSY186 |
| JSY285 | msh4∷kanMX4 | HMY223 × JSY249 |
| JSY292 | mlh1∷hygB mlh2∷kanMX4 | JSY181 × JSY259 |
| JSY296 | msh5∷hygB | JSY293 × JSY295 |
| JSY313 | pol3-01 | JSY307 × JSY308 |
| MW103 | Wild type | AS4 × MW1 (Nag et al. 1989) |
| Other diploids | ||
| JSY276 | arg4-tel pms1∷hygB | JSY269 × JSY270 |
| JSY282 | arg4-tel msh2∷kanMX4 | JSY279 × JSY278 |
| MW160 | arg4-tel | MW81 × MW79 (White et al. 1993) |
| MC153 | Wild type | AS4 × AS4 [created by inducing HO expression on the plasmid pGAL-HO (Herskowitz and Jensen 1991) in AS4 and then selecting for a diploid that had lost the plasmid] |
| MC156 | Wild type +pDJ173, plasmid-borne STP22 URA3 | MW153, carrying pDJ173 (Li et al. 1999) |
a
Genotypes of the haploids used in the constructions are given in Table 1. In crosses, AS4- and AS13-derived strains are shown at the left and the right of the ×, respectively.