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. 2006 Jul;173(3):1223–1239. doi: 10.1534/genetics.106.055616

TABLE 6.

Genetic distances in three genetic intervals on chromosome III

HIS4–LEU2
LEU2–CEN3
CEN3–MAT
Strain(s)a Relevant genotype P N T CMb P N T CMc P N T CMc
PD83/MW103 Wild type 286 8 168 24 358 338 165 10 209 185 364 24
JSY240 msh2Δ 73 3 37 24 70 74 33 9 40 45 82 25
JSY163/JSY188 msh3Δ 104 4 81 28 157 162 78 10 91 95 168 24
JSY291/JSY285 msh4Δ 172 2 49 14d 129 113 47 8 107 112 88 14e
JSY297/JSY296 msh5Δ 141 0 31 9d 112 106 40 8 106 99 55 11e
JSY164/JSY216 msh6Δ 120 3 87 25 132 161 78 11 70 82 154 25
JSY349 msh2Δ msh4Δ 90 1 17 11d 81 83 33 8 76 71 40 11e
HMY95/JSY230 mlh1Δ 220 3 77 16d 261 258 125 10 142 155 121 14e
JSY165/JSY210 mlh2Δ 155 5 87 24 138 168 61 8 97 108 162 22
JSY178/JSY216 mlh3Δ 133 4 81 24 168 155 50 7 148 123 140 17e
HMY96/JSY233 pms1Δ 226 1 128 19 325 336 117 8 136 116 192 22
JSY277 mlh1Δ pms1Δ 210 0 70 13d 159 147 62 8 127 102 108 16e
JSY292 mlh1Δ mlh2Δ 99 0 25 10d 60 75 33 10 53 75 46 13e
JSY283 mlh1Δ mlh3Δ 180 3 74 18 130 163 70 10 120 142 117 15e
JSY136/JSY284 exo1Δ 223 2 69 14d 170 166 30 4f 132 139 84 12e
JSY314/JSY313 pol3-01 85 5 43 27 55 63 38 12 35 43 77 25
JSY255 rad1Δ 82 7 56 34 85 91 23 6 74 46 79 20
JSY239 rad27Δ 127 2 54 18 96 93 39 9 36 36 77 26
JSY320 bas1Δ 163 1 63 15 93 95 54 11 67 75 110 22
JSY321 bas1Δ msh2Δ 108 1 45 17 77 78 41 11 55 47 86 23
JSY338 his4-51 195 5 65 18d 130 109 53 9 101 69 135 22
JSY343 his4-51 msh2Δ 184 11 102 29 131 100 74 12 78 70 151 25

P, N, and T indicate parental ditype (PD), nonparental ditype (NPD), and tetratype tetrads (T), respectively. We included only those tetrads in which both flanking markers had Mendelian segregation.

a

For some intervals, data from multiple strains were pooled. These strains were isogenic, except for the specific mutations at the HIS4 locus.

b

Calculated by the equations of Perkins (1949).

c

Calculated as the percentage of second-division segregation (tetratype tetrads) divided by two. First-division segregation (FDS) and second-division segregation (SDS) tetrads were determined using the heterozygous centromere-linked trp1 marker.

d

Significant difference compared to the wild-type strain (PD83) in the numbers of PD:NPD:T tetrads. The P-values were determined using chi-square analysis. The P-values for each of the 21 comparisons were ranked and the Benjamini and Hochberg (1995) procedure was applied using an initial α = 0.05. P-values <0.019 were considered significant.

e

Significant difference compared to the wild-type strain (PD83) in the numbers of FDS:SDS tetrads for the CEN3–MAT interval. The P-values were determined using two-tailed Fisher exact tests. The P-values for 21 comparisons were ranked and the Benjamini and Hochberg (1995) procedure was applied using an initial α = 0.05. P-values <0.021 were considered significant.

f

Significant difference compared to the wild-type strain (PD83) in the numbers of FDS:SDS tetrads for the LEU2–CEN3 interval. The P-values were determined using two-tailed Fisher exact tests. The P-values for 21 comparisons were ranked and the Benjamini and Hochberg (1995) procedure was applied using an initial α = 0.05. P-values <0.002 were considered significant.