Figure 2. Functional Responses of Murine CD8+ T Cells Directed against Non-ts Epitopes.
(A) SCs from naive (gray bars) or chronically infected (Brazil strain; black bars) B6 mice were stimulated for 5 h with TSKB20, cruzipain, or GFT peptides plus GolgiPlug as described in Materials and Methods. Data represent the number of CD8+ T cells producing IFNγ; error bars represent SD.
(B) SCs from naive B6 mice were loaded with T. cruzi peptides and labeled with CFSE as described in Materials and Methods. The numbers above the peaks represent the percentage of specific killing of the target cells loaded with the indicated peptide and was calculated as described in Materials and Methods. Data are representative of three experiments.