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. 2006 May 23;6:22. doi: 10.1186/1471-213X-6-22

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Copyright © 2006 Kallio et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunostaining of CA IX and CA XII in the kidney of E12.5 mouse embryos and in the adult mouse kidney. Both CA IX (A) and CA XII (B) show weak staining in the ductal epithelium of the embryonic tissue, and a positive immunoreaction is seen for both isozymes in the adult mouse renal tubules (C, D), with CA XII also located in the collecting ducts. Control immunostaining of an adult mouse kidney with NRS gave no positive signal (E). Manual PAP staining in panels A-B and E, automated immunostaining in panels C-D. Original magnifications: A-B × 400, C-E × 100.