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. Author manuscript; available in PMC: 2007 Aug 3.
Published in final edited form as: Oncogene. 2006 Jun 19;25(33):4559–4572. doi: 10.1038/sj.onc.1209462

Figure 1.

Figure 1

Expression levels of the 14-3-3 proteins in prostate cancer cell lines.
  1. Immunoblot analysis of 14-3-3 proteins in prostate cancer cell lines PC3, DU145, LNCaP and Tramp-C1. Equal amounts of total cellular protein lysates extracted from each of the cell lines were electrophoresed on SDS-PAGE, subjected to immunoblot with anti-14-3-3β, anti-14-3-3ξ, anti-14-3-3ε, anti-14-3-3σ, and anti-GAPDH antibodies, and followed by chemiluminescence detection. GAPDH was used as a loading control.
  2. Immunostaining for 14-3-3σ protein expression in PC3, DU145, LNCaP and Tramp-C1 cells with anti-14-3-3σ antibodies (red signal). Nuclei were stained with DAPI (blue signal).