Figure 1.

A cDNA-AFLP Autoradiogram Showing the Accumulation Patterns of TDFs from Zinnia Cultured Cells during TE Formation.

(A) Templates were derived from Zinnia cells cultured for 48 h in medium with no growth factors (lane 0) and 0.5, 4, 24, and 48 h after the addition of auxin and cytokinin. Lanes are in groups of five: each group was amplified using a different primer combination with two selective nucleotides.

(B) Enlarged view of the boxed region in (A). Various expression patterns can be detected across the time course: constitutive expression (i), downregulation (ii), transient expression (iii), and upregulated expression (iv).