Figure 3.

Comparison of Expression Patterns of Selected Genes by Both cDNA-AFLP and RT-PCR.

(A) Time course of Zinnia mesophyll cells transdifferentiating into TEs, and the corresponding number of ESTs first accumulated at each time point.

(B) and (C) Comparison of expression patterns of 12 cDNA-AFLP fragments, similar to genes known to be involved in vascular differentiation, by cDNA-AFLP (B) and RT-PCR (C). RNA templates were derived from Zinnia cells cultured in noninductive medium for 48 h (lane 0) and cells left for 30 min, 4 h, 24 h, and 48 h (lanes 0.5, 4, 24, and 48, respectively) after the addition of auxin and cytokinin. Ze203 (MONOPTEROS-like), Ze519 (ZCAD1-like), and Ze414 (CSL-like) transcripts were present already before the addition of auxin and cytokinin. Ze349 (PIN1-like), Ze622 (REVOLUTA/IFL1-like), Ze572 (TED3-like), Ze370 (PEL1-like), and Ze293 (EXP1-like) were upregulated at the 4-h time point. The mRNAs for genes similar to Ze452 (CESA-like), Ze374 (TED4-like), Ze68 (TED2-like), and Ze94 (LACCASE-like) were upregulated at later stages of the developmental pathway.