Expression of Ze469, Ze126, and Ze86 cDNAs in Cells Cultured in Vitro.
(A) The lanes contain total RNA from cells cultured in noninductive medium for 48 h (lane 1), from cells at 30 min and 4, 24, 48, 60, and 72 h (lanes 2, 3, 4, 5, 6, and 7, respectively) after the addition of auxin and cytokinin at 48 h, from cells in medium containing 1.0 mg/L auxin but no cytokinin for 72 and 96 h (lanes 8 and 9), and from cells in medium containing 1.0 mg/L cytokinin but no auxin for 72 and 96 h (lanes 10 and 11). The corresponding Zinnia accession numbers are presented at the end of Methods.
(B) cDNA-AFLP expression patterns of Ze469, Ze126, and Ze86. The Zinnia cells were cultured with auxin and cytokinin for the times indicated above the gels (in hours) before harvesting.