Figure 4.

One-Dimensional SDS-PAGE Analysis of Seed Protein Content during Development and Germination.

(A) Mature or germinating seed (24 h) protein from the wild type (C) or homozygous βVPE::dSpm1 (β), homozygous δVPE::dSpm1 (δ), or homozygous βVPE/δVPE double mutants (δ/β). Arrowheads indicate observed differences. The approximate gel locations of legumin-type proglobulins (pro), α- and β-chains, and napin-type large and small chains are shown with brackets.

(B) Seed protein extracted from the wild type (C) or homozygous βVPE::dSpm1 (β) at 6, 9, 12, and 15 DAA and maturity (M). Arrowheads indicate observed differences.

(C) Protein extracted from germinating wild-type (C) or homozygous βVPE/δVPE double-mutant (δ/β) seeds. After cold treatment at 4°C for 48 h, seeds were incubated at 25°C for 0, 24, and 48 h.