Table 1.
Mass Spectrometric Identification of Seed Protein Features That Were Changed in Accumulation between Homozygous βVPE::dSpm1 and Wild-Type Seeds
| ID No.a | pI | kD | % WT b | % βVPE b | Change c | t Test d | Annotation Detail | PIDe |
|---|---|---|---|---|---|---|---|---|
| 52_6.9 | 6.88 | 52171 | 0.001 | 0.044 | 33.4 | 0.05 | Vicilin-like precursor f | 9279583 |
| 51_7.2 | 7.15 | 51335 | 0.022 | 0.233 | 10.7 | 0.08 | Vicilin-like precursor f | 9279583 |
| 50_8.1 | 8.07 | 49734 | 0.000 | 0.033 | NAg | 0.03 | Vicilin-like precursor f and legumin-type globulin precursor f |
9279583 1628583 |
| 49_7.4 | 7.39 | 49332 | 0.052 | 0.135 | 2.6 | 0.08 | Vicilin-like precursor f and catalase 2 |
9279583 1705620 |
| 49_7.2 | 7.15 | 49412 | 0.025 | 0.100 | 4.0 | 0.08 | Vicilin-like precursor f | 9279583 |
| 48_7.8 | 7.84 | 47906 | 0.038 | 0.705 | 18.6 | 0.12 | Legumin-type globulin precursor f | 1628583 |
| 48_7.6 | 7.61 | 48268 | 0.011 | 0.115 | 10.3 | 0.15 | Legumin-type globulin precursor f | 1628583 |
| 47_7.1 | 7.09 | 46509 | 0.007 | 0.117 | 16.8 | 0.11 | Legumin-type globulin precursor f | 9759513 |
| 46_6.9 | 6.92 | 45515 | 0.003 | 0.023 | 6.7 | 0.03 | Legumin-type globulin precursor f | 1628583 |
| 46_5.4 | 5.39 | 46101 | 0.011 | 0.210 | 18.6 | 0.11 | Legumin-type globulin precursor f | 4204298 |
| 45_6.7 | 6.69 | 45347 | 0.076 | 0.724 | 9.5 | 0.01 | Legumin-type globulin precursor f | 4204299 |
| 35_7.9 | 7.88 | 34774 | 0.040 | 0.095 | 2.4 | 0.23 | Legumin-type globulin precursor f,h | 9759513 |
| 31_5.9 | 5.85 | 30545 | 0.016 | 0.034 | 2.1 | 0.01 | Legumin-type globulin precursor f,h | 9759513 |
| 30_6.7 | 6.74 | 29573 | 0.542 | 0.118 | −4.6 | 0.02 | α-chain of legumin-type globulinf,h | 1628583 |
| 30_6.2 | 6.20 | 29787 | 0.350 | 0.099 | −3.5 | 0.01 | α-chain of legumin-type globulinf,i | 9759513 |
| 29_6.6 | 6.60 | 29117 | 6.057 | 2.154 | −2.8 | 0.50 | α-chain of legumin-type globulinf,i | 9759513 |
| 28_6.8 | 6.76 | 27722 | 0.975 | 2.559 | 2.6 | 0.08 | Legumin-type globulin precursor f,h | 4204299 |
| 26_7.54 | 7.54 | 25955 | 0.000 | 0.614 | NA | 0.10 | α-chain of legumin-type globulinf,h | 9759513 |
| 26_5.4 | 5.36 | 25569 | 0.068 | 0.200 | 2.9 | 0.06 | α-chain of legumin-type globulinf,i | 4204298 |
| 25_7.7 | 7.66 | 25357 | 0.033 | 0.000 | NA | 0.01 | α-chain of legumin-type globulinf,i | 4204299 |
| 25_6.8 | 6.76 | 24819 | 0.148 | 0.387 | 2.6 | 0.10 | α-chain of legumin-type globulinf,i | 4204299 |
| 24_6.3 | 6.26 | 24249 | 0.066 | 0.160 | 2.4 | 0.00 | α-chain of legumin-type globulinf,h | 4204299 |
| 20_7.8 | 7.77 | 19736 | 0.105 | 0.005 | −22.8 | 0.00 | β-chain of legumin-type globulinf,i | 1628583 |
| 20_7.3 | 7.27 | 19614 | 0.006 | 0.223 | 39.8 | 0.07 | β-chain of legumin-type globulin with N-terminalf,h extension |
9759513 |
| 19_11.9 | 11.92 | 19146 | 0.092 | 0.019 | −4.9 | 0.34 | β-chain of legumin-type globulinf,i | 1628583 |
| 18_8.5 | 8.50 | 17673 | 0.152 | 1.342 | 8.8 | 0.12 | β-chain of legumin-type globulin with N-terminalf,h extension |
1628583 |
| 17_5.8 | 5.81 | 17598 | 0.039 | 0.113 | 2.9 | 0.03 | β-chain of legumin-type globulinf,h | 4204299 |
| 16_7.7 | 7.68 | 16299 | 0.006 | 0.117 | 18.5 | 0.06 | β-chain of legumin-type globulinf,h | 1628583 |
| 13_8.9 | 8.87 | 13231 | 0.051 | 0.145 | 2.9 | 0.09 | α-chain of legumin-type globulinf,h | 9759513 |
| 13_7.9 | 7.93 | 13337 | 0.030 | 1.463 | 49.1 | 0.00 | Napin-type albumin 2S 3 precursor f | 166616 |
| 11_5.2 | 5.18 | 11198 | 0.082 | 0.026 | −3.1 | 0.24 | Embryo-specific protein 3 | 7546697 |
| 11_5.0 | 5.00 | 10615 | 0.150 | 0.057 | −2.6 | 0.00 | α-chain of legumin-type globulinf,h | 1628583 |
| 11_4.9 | 4.88 | 11118 | 0.165 | 0.513 | 3.1 | 0.03 | α-chain of legumin-type globulinf,h | 1628583 |
| 11_4.7 | 4.65 | 11111 | 0.051 | 0.118 | 2.3 | 0.03 | α-chain of legumin-type globulinf,h | 1628583 |
Identification number assigned for reference to Figure 6.
Percentage of integrated fluorescence intensity of a specific protein spot in relation to the integrated fluorescence intensity of all detected protein spots.
Fold increase (positive numbers; calculated as the quotient of % βVPE and % WT) or fold decrease (negative numbers; calculated as the quotient of % WT and % βVPE) of the integrated fluorescence intensity of a particular protein spot in βVPE mutant seeds compared with wild-type seeds. This calculation was not applicable (NA) when a specific protein spot was not detected in both samples.
Significance of comparisons between three gels each of wild-type and βVPE mutant seeds.
PID, protein identification number in GenBank.
Mass spectrometry coverage data were used to further suggest the relationship of the individual polypeptide fragment with respect to its corresponding full-length protein form.
NA, not applicable.
Legumin-type globulin polypeptides identified as alternatively processed forms (see Results for details).
Legumin-type globulin polypeptides identified as normally processed or redundantly processed forms (see Results for details).