Figure 7.

I-2 and Mi-1 Exhibit ATPase Activity.

I-2N (1.9 μM; wild type [wt]) and I-2N^K207R (1.9 μM; mutant [mut.]), together with a buffer control (A) or 1.5 μM Mi-(161-900) (B), were incubated with 5.0 μM α-³²P-ATP (4.4 × 10⁴ cpm/pmol). Reactions were subjected to thin layer chromatography to detect the production of α-³²P-ADP with autoradiography and quantitated by phosphorimaging as described in Methods.