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. 2003 Apr 1;31(7):1921–1934. doi: 10.1093/nar/gkg280

Figure 2.

Figure 2

Magnesium dependence of the suicide inhibition (S.I.) and reverse cyclization (R.C.) reactions. (A) A typical polyacrylamide gel of the reactions. Lanes 0–15 show reactions using 1 nM radiolabeled 5′ exon mimic d(ATGAC)rU and 500 nM of rP-h in HXMg buffer [50 mM HEPES (25 mM Na+), 135 mM KCl and X mM MgCl2 (from 0 to 15) at pH 7.5]. Lanes A and B show reactions using 1 nM radiolabeled 5′ exon mimic Pr2-d(ATGAC)rU (27mer) and 500 nM of rP-h in H4Mg (lane A) or H15Mg (lane B) buffer. All reactions were run for 2 h, which allows maximum product formation. The sizes of the 27, 52, 350 and 370mer are approximate. (B) Graph of the MgCl2 concentration dependence for the suicide inhibition (open squares) and the reverse cyclization (solid circles) reactions shown above. Note that both reactions occur simultaneously and that the graph is an average of two reactions.