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. 2003 Apr 1;31(7):1921–1934. doi: 10.1093/nar/gkg280

Figure 3.

Figure 3

Competitive binding assay with d(ATGAC)rU in 50 mM HEPES (25 mM Na+), 15 mM MgCl2 and 135 mM KCl at pH 7.5. (A) A native polyacrylamide gel showing the partitioning of bound and free radiolabeled r(AUGACU) as a function of non-radiolabeled d(ATGAC)rU concentration. Reactions utilized 30 nM rP-8/4x ribozyme, 1 nM radiolabeled 5′ exon mimic r(AUGACU), and 5–1500 nM of the competitor d(ATGAC)rU. (B) Plot and curve-fit of the data in (A). Note that the results in Table 4 are an average of two independent assays.