Table 1. Suicide inhibitors of the self-splicing reaction.

(A) Suicide inhibitors obtained from the combinatorial assays (written 5′→3′). The number of occurrences for each sequence is indicated in the left column for reactions at 4 mM MgCl₂ and in the right column for reactions at 15 mM MgCl₂. Note that position –1 is RNA and was not randomized, while positions –2 through to –6 are DNA and were randomized. The numbering system for the inhibitor positions is shown above the sequences. Note that the inhibitors are 5′ exon mimics. (B) Sequence of the IGS of the rP-h intron (and the rP-8/4x ribozyme). (C) The percent occurrence of the four nucleobases at each position. Note that the percent occurrences are different for the two MgCl₂ concentrations.