tRNAIle precursor 3′ end processing of wild-type and selected pathological mutants. 5′ End labeled tRNAs were processed without added unlabeled tRNA. (A) Wild-type, (B) A4269G, (C) G4309A, (D) A4317G. Reactions were sampled after 0, 5, 10 and 15 min incubation at 37°C in lanes 1–4 of each set. M is same as in Figure 2.