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. 2003 Apr 1;31(7):1877–1887. doi: 10.1093/nar/gkg300

Figure 5.

Figure 5

hImp3 and hImp4 interact with hMpp10 but not with each other in vitro. Radiolabeled hMpp10, hImp3 and hImp4 were expressed in an in vitro translation system (lanes 1–3). These polypeptides were incubated with GST-tagged hImp3 or hImp4 as indicated, followed by precipitation with immobilized glutathione (lanes 4–9). A GST15.5K fusion protein was used as a control (lane 10). Note that hImp4 is only co-precipitated with GSThImp3 in the presence of hMpp10 and that, vice versa, hImp3 is only co-precipitated with GSThImp4 in the presence of hMpp10. The positions of radiolabeled hMpp10, hImp3 and hImp4 in the gel are indicated on the right. On the left of each panel the positions of molecular mass markers are shown.