Figure 1.

Scheme of the AMEGA system. (A) Chimeric receptor constructs used in this study. The extracellular N-terminal half of EpoR was replaced with either the V_H or V_L region of HyHEL-10 with a Gly-Ser-Gly tripeptide linker between V_H/V_L and Val118 of the EpoR D2 domain. The transmembrane and intracellular domains could be easily replaced at the EcoRV site inserted at the C-terminus of the EpoR D2 domain. (B) Schematic diagram of the selection procedure. Chimeric receptor combination of Hg and LE is depicted in the scheme as a representative. Retroviral vectors with long- terminal repeats (LTRs) and a packaging signal (ψ) are used for the efficient transcription of the Hg and LE-IRES-transgene. An immunoglobulin heavy-chain secretion signal sequence (S) is located upstream of the chimeric receptor genes to enable their cell surface expression.