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. 2003 Apr 1;31(7):e32. doi: 10.1093/nar/gng032

Figure 2.

Figure 2

Antigen-mediated expansion of the transfectants with chimeric receptor expression. The expression level of the chimeric receptor was compared before (bef.) and after (aft.) selection. Selection was performed in the culture medium containing either no factors (–), 1 µg/ml HEL or 2 ng/ml IL-3. pMX means Ba/F3 cells transfected with pMX vector without any inserts (named Ba/pMX) as a negative control. (A) Hg+Lg combinations, (B) HE+Lg combinations and (C) Hg+LE combinations were analyzed with anti-gp130 and anti-EpoR C-terminus antibodies. In each lane, cell lysate from 1.1 × 105 cells was loaded. The actual dates for the harvest were as follows. In (A), day 6 for HgIGFP, Lg and HgIGFP+Lg bef.; day 39 for aft. (–) and HEL; day 36 for IL-3. In (B), day 7 for LgIGFP, HE and HE+LgIGFP bef.; day 67 for aft. (–); day 37 for HEL; day 56 for IL-3. In (C), day 6 for HgIGFP; day 7 for LEIGFP and Hg+LEIGFP bef.; day 40 for aft. HEL; day 56 for IL-3; day 6 for HgIGFP+LE bef.; day 39 for aft. HEL; day 36 for IL-3.