Figure 1.

Comparative analysis of the T4 PNK with l-DNA 1–4 (the terminal nucleotides are indicated). 400 pmol of the respective l-DNA was incubated in kinase buffer with 40 U T4 PNK in the presence of [γ-³²P]ATP in a 20 µl reaction. Incubation was carried out at 37°C for 3 h. Products were visualized by autoradiography (20% denaturing polyacrylamide gel).