Table 1. Yield of kinase reaction for l-DNA 15mers 1–4 (incorporation of the 32P label, two independent labeling experiments).
Terminal nucleotide (l-DNA 1–4) | G (1) | C (2) | A (3) | T (4) |
---|---|---|---|---|
Yield, labeling 1 (%) | 43.9 | 46.8 | 27.6 | 31.0 |
Yield, labeling 2 (%) | 43.5 | 49.3 | 25.9 | 30.1 |
Average yield (%) | 44 | 48 | 27 | 31 |
The yield of each phosphorylation reaction was calculated by dividing the radioactivity of the respective labeled oligonucleotide (excised band from gel) by the input radioactivity.