Table 3. Detection probability: different RNases and modified transcripts; probability (%) of detecting one SNP in 1000 bp.
| PCR product size | RNase T1 α-thio CTP | RNase T1 2′-F-UTP | RNase A | RNase A 2′-F-UTP | RNase A 2′-F-CTP | Combination T1 α-thio/A 2′-F |
|---|---|---|---|---|---|---|
| 1000 | 70.6 | 51.2 | 16.7 | 79.4 | 79.1 | 92.2 |
| 500 | 83.4 | 64.1 | 24.4 | 88.2 | 87.3 | 95.3 |
| 333 | 89.3 | 71.3 | 30.1 | 91.1 | 90.8 | 95.7a |
| 250 | 91.2 | 76.5 | 34.8 | 93.0 | 92.4 | 95.8 |
| 200 | 92.9 | 79.8 | 37.9 | 94.2 | 93.2 | 95.9 |
| 100 | 94.7 | 87.6 | 48.5 | 95.0 | 94.5 | 95.9 |
aA detection rate of 96% has to be considered as the theoretical maximum, as 4% of the 1000 bp are occupied by the first and last primer and are therefore not sensitive for mutations.