Figure 5.
Nucleotide frequencies at targetron insertion sites obtained using a RAM-targetron with randomized EBS and δ sequences. The figure summarizes nucleotide frequencies (%) at positions –30 to +15 in 90 different insertion sites in the E.coli genome. Strongly favored or disfavored nucleotide residues are highlighted: green, N > 50%; yellow, S (G + C) > 75%; blue, Y (C + T) > 75%; pink, N < 5%. We found no additional strongly favored or disfavored nucleotide residues from position –100 to +100 from the targetron insertion site.
