Table 1. Fractional saturation values.
| GAC | BRCA1 | p16INK4a | MLH1 | GSTP1 | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GAM1 (M) | GAC (U) | U/M | M | U | U/M | M | U | U/M | M | U | U/M | M | U | U/M | |
| xMeCP2 | 48.2 ± 8.5 | 555.9 ± 77.3 | 11.5 | 21.7 ± 7.7 | 394.6 ± 99.7 | 18.2 | 57.6 ± 15.4 | – | – | 61.3 ± 11.9 | 473.2 ± 117.6 | 7.7 | 43.8 ± 16.0 | 527.7 ± 106.1 | 12.0 |
| xMBD3 | 186.5 ± 42.5 | 778.6 ± 256.8 | 4.2 | 62.9 ± 15.9 | 555.0 ± 127.7 | 8.8 | 90.5 ± 15.4 | – | – | 121.6 ± 23.5 | 676.6 ± 250.3 | 5.6 | 28.8 ± 7.2 | 536.9 ± 162.7 | 18.6 |
| mMeCP2 | 171.8 ± 23.2 | 458.4 ± 88.2 | 2.7 | 161.3 ± 39.9 | 441.0 ± 138.5 | 2.7 | 150.5 ± 23.2 | – | – | 192.0 ± 49.1 | 640.0 ± 49.3 | 3.3 | 118.5 ± 25.3 | 349.2 ± 87.8 | 2.9 |
| mMBD3 | 579.5 ± 105.3 | 683.81 ± 124.3 | 1.1 | – | – | – | – | – | – | - | – | – | – | – | – |
| mMBD2b | 2.7 ± 0.8 | 188.7 ± 46.8 | 69.8 | 3.5 ± 1.5 | 200.4 ± 53.6 | 57.3 | 1.6 ± 0.2 | – | – | 7.9 ± 1.9 | 257.4 ± 125.4 | 32.5 | 1.09 ± 0.1 | 136.5 ± 37.3 | 125.2 |
| xMBDR LF | n.b. | n.b. | – | n.b. | n.b. | - | n.b. | – | – | n.b. | n.b. | – | n.b. | n.b. | – |
R1/2 values for Xenopus (x) and mammalian (m) MBD proteins and synthetic oligos (GAC, BRCA1, p16INK4a, MLH1 and GSTP1) in their methylated (M) and unmethylated (U) forms calculated by fitting the data obtained from R-CEMSA to non-cooperative binding behavior using GraFit software. Ratios of R1/2 unmethylated values and R1/2 methylated values are shown (U/M). Results are expressed as mean ± SD. n.b., no-retardation peak detected. Values are expressed in molar units and are multiplied by 109.