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. 2003 Mar 15;31(6):1683–1692. doi: 10.1093/nar/gkg258

Figure 1.

Figure 1

Spot assay. The S.kluyveri strain Y777, deficient in pyd2, was transformed with putative PYD2 genes from different eukaryotes, giving the following transformants: P478 (containing the A.thaliana PYD2 gene, At), P633 (containing the D.discoideum PYD2 gene, Dd) and P635 (containing the D.melanogaster PYD2 gene, Dm). The growth on the ‘rich’ medium, SD+uracil, and two selective media, SD and DHU is shown. Y156 is the parental strain of Y777, and both are uracil auxotrophs. Note that the plasmids also contain, apart from PYD2, the URA3 gene.