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Reaction mechanism of DHPase. The V/K for DHU decreases at both low and high pH giving pK values of about 7.5–8.0 and 9–10. The enzymic general base (most likely D326) with a pK of 7.5–8.0 is required to activate the water for nucleophilic attack on the C-4 of DHU which is directly coordinated to the active site zinc. The second group with a pK of 9–10 likely reflects Zn-water ionization of the free enzyme.
