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| Sk | Dd | |
|---|---|---|
| Native molecular massa (kDa) | 255 | 220 |
| Subunit molecular mass (kDa) | 60 | 56 |
| Metal contentb per subunit | 2 Zn | 2 Zn |
| Substrate specificity | DHU, DHT | DHU, DHT |
| Glutarimide (very slowly) | ||
| NCBA | NCBA |
aThe native size was determined by native gel electrophoresis (see also Supplementary Material).
bMetal determination on Dd and Sk DHPases after removal of all metal ions and subsequent saturation with zinc ions.
