Table 2. Kinetic parameters of DHPases from various organisms.
Enzyme | Substrate | Km (µM) | Vmax (µM min–1 mg–1) | Vmax/Km (min–1 mg–1) |
---|---|---|---|---|
S.kluyveri | DHU | 710 ± 90 | 1340 ± 85 | 1.88 |
DHT | 490 ± 80 | 700 ± 30 | 1.43 | |
NCBAa | 10 000 ± 250 | 160 ± 20 | 0.016 | |
D.discoideum | DHU | 400 ± 50 | 550 ± 20 | 1.38 |
DHT | 37 ± 5 | 176 ± 6 | 4.8 | |
NCBAa | 2400 ± 250 | 76 ± 2 | 0.032 | |
Calf liver | DHU | 25 | 3670 | 146.8 |
DHT | 85 | 8341 | 98.1 | |
NCBAb | 4100 | 1500 | 0.36 |