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. 2003 Mar 15;31(6):1683–1692. doi: 10.1093/nar/gkg258

Table 2. Kinetic parameters of DHPases from various organisms.

Enzyme Substrate Km (µM) Vmax (µM min–1 mg–1) Vmax/Km (min–1 mg–1)
S.kluyveri DHU 710 ± 90 1340 ± 85 1.88
  DHT 490 ± 80 700 ± 30 1.43
  NCBAa 10 000 ± 250 160 ± 20 0.016
D.discoideum DHU 400 ± 50 550 ± 20 1.38
  DHT 37 ± 5 176 ± 6 4.8
  NCBAa 2400 ± 250 76 ± 2 0.032
Calf liver DHU 25 3670 146.8
  DHT 85 8341 98.1
  NCBAb 4100 1500 0.36

The data for the calf liver DHPase are taken from Jahnke et al. (12).

apH 6.5.

bpH 5.5.