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. 2003 Mar 15;31(6):1605–1613. doi: 10.1093/nar/gkg259

Figure 5.

Figure 5

Denaturing gel electrophoresis of the products from the 3′-exonuclease digestion of (T2AG3)4 II and its platinated products from reaction with trans-[Pt(NH3)2(H2O)2]2+ 2 (2.1–2.4 from Fig. 3). The digestion reaction was conducted with different enzyme dilutions indicated at the top of each lane (e.g. 1 = none, 5 = five times, 30 = 30 times dilution). The numbered purines correspond to the digestion arrests. They were determined from the migration of the corresponding fragments after deplatination by NaCN. The digestion of II gives the reference scale for the migration of the platinated digested fragments. For the sake of comparison, we have adopted the same base numbering for the 22mer I and the 24mer II (Fig. 1).