Fig. 5. Presence of HMGN1 on the chromatin of Hmgn2 and Dhfr genes. (A) Primers used to detect the genes in the ChIP assays. Black boxes indicate exons. (B) PCR analysis of chromatin immunoprecipitations from Hmgn1^–/– and Hmgn1^+/+ cells with antibodies to HMGN1. Note the lack of signals in the IP DNA from Hmgn1^–/– cells. This analysis is not quantitative. (C) Quantification, by real time PCR analysis, of the Hmgn2 and Dhfr genes in IP from Hmgn1^+/+ cells. The value for β-globin is set to 1 and the bar graphs represent the average of three experiments.