Table II. Average nucleosome length (La) following micrococcal nuclease digestion.
Ethidium bromide |
Autoradiogram |
|||||
---|---|---|---|---|---|---|
Hmgn1+/+ | Hmgn1–/– | Hmgn1–/–/Hmgn1+/+ | Hmgn1+/+ | Hmgn1–/– | Hmgn1–/–/Hmgn1+/+ | |
La – lane 4a | 2.88 | 2.91 | 1.01 | 1.78 | 2.29 | 1.29 |
La – lane 5a | 1.42 | 1.46 | 1.02 | 1.66 | 2.02 | 1.22 |
Averageb | 1.02 ± 0.01 | 1.46 ± 0.2 |
aLanes from Figure 6.
bAverage from three different experiments.
La, average nucleosome length was calculated as: La = ΣN1–N6 (PN). Where N1–N6 is the oligonucleosome size (i.e. mono, di) and PN is the fraction of a particular oligonucleosome size out of the total scan (the region covering mono- to hexa-nucleosomes was scanned).
Statistical significance of the two groups was determined by Student’s t-test, p < 0.01.