Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Fig. 6. Schematic representation of plasmid pET21b derivatives expressing C-terminally His-tagged eRF1 from human (pET-Hs-eRF1-His6) or Euplotes (pET-Eu-eRF1-His6) under the control of the T7 promoter (T7p). The restrictions sites used for the construction of the recombinant eRF1s are indicated. The restriction sites indicated under pET-Hs-eRF1-His6 were introduced by site-directed mutagenesis.
