Fig. 5. The ompL null mutants are not resistant to bacteriophage M13 infection. Lawns of isogenic derivatives of JM101 were prepared on L-agar plates in the presence of IPTG and X-gal. Ten-fold serial dilutions of bacteriophage M13mp7 were spotted on the lawns followed by incubation of the plates at 37°C for ∼18 h. A representative set of results obtained with the parent JM101 (WT), the dsbA and ompL single mutants and the dsbA ompL double mutant is shown. The ompL allele shown here is the ompL::mini-Tn10Kan^R allele (Dartigalongue et al., 2000).