Fig. 4. The binding of Rap1 to the T₂AG₃-only telomere is impaired. A myc tag epitope was inserted at the C-terminus of the RAP1 endogenous gene in Y, Y tel1Δ and Hy strains carrying an URA3-tagged telomere. (A) Schematic representation of the three DNA fragments analyzed for Rap1 binding by ChIP and by real-time PCR. (B) Results of the immunoprecipitation are expressed as the ratio of the immunoprecipitated TEL DNA to that of the RPG DNA. The average value for at least three experiments is given in the diagram as well as the estimated standard deviation.