Fig. 6. SiRNA-mediated annexin 2 gene silencing inhibits channel activity in HEK293 cells heterologously expressing TRPV5. Immunoblot showing annexin 2 (A), S100A10 (B), TRPV5 and TRPV6 (C) protein levels in control (CT) and annexin 2-specific siRNA-treated HEK293 cells. Images (E) and corresponding histogram (D) of annexin 2 immunofluorescence staining of control and annexin 2-specific siRNA transfected HEK293 cells. Ca²⁺ currents in response to a voltage step from +20 to –100 mV in control and annexin 2-specific siRNA transfected HEK293 cells (F). Current response to a voltage ramp from –150 to 100 mV measured in NMDG⁺ and divalent-free solution in control and annexin 2-specific siRNA transfected HEK293 cells (G). Distribution of Na⁺ currents as a measure of channel activity analyzed in control (open bars) and annexin 2-specific siRNA-transfected (closed bars) HEK293 cells at –80 mV (H) and the corresponding averaged data (I). N is more than 25 cells of two independent transfections. *P < 0.001 significantly different from control. CT, control.