Table 1.
Induction of Side Branches on No-Branched Protonema of Cryptochrome Disruptants under Different Light Conditions
| Number of Induced Branches (n)
|
||
|---|---|---|
| Strain | Blue Light (28 μmol m−2·sec−1) |
Red Light (26 μmol m−2·sec−1) |
| Wild type | 2.15 ± 0.16 (106) | 0.88 ± 0.08 (104) |
| cry1a-1 | 0.65 ± 0.14 (40) | 0.68 ± 0.15 (37) |
| cry1a-2 | 0.63 ± 0.10 (81) | 0.72 ± 0.17 (32) |
| cry1b-1 | 0.41 ± 0.14 (29) | 0.69 ± 0.15 (26) |
| cry1b-2 | 0.29 ± 0.16 (21) | 0.75 ± 0.14 (40) |
| cry1a cry1b-1 | 0.08 ± 0.03 (74) | 0.50 ± 0.07 (103) |
| cry1a cry1b-2 | 0.02 ± 0.02 (62) | 0.80 ± 0.13 (74) |
Protonemata were precultured between cellophane and a cover slip on agar medium under unilateral red light (2.8 μmol m−2·sec−1) for 9 days. Protonemata of more than 1.5 mm in length, having no branch, were used for this experiment. Stimulus light was given continuously for 2 additional days, and the number of branches induced was scored. Two-day dark incubation did not produce any side branches on wild-type protonemata (n = 97).
Values shown are means ±se; n indicates number of protonemata counted.