Table 2.
Effects of Taxol on Neocentromere Number in Ab10 Heterozygotes and N10 Segregants
| Fixeda
|
Cultured without Taxol
|
Cultured with Taxol
|
||||
|---|---|---|---|---|---|---|
| Genotypeb | AIc | AII | AI | AII | AI | AII |
| N10/N10 Mean no. of neocentromeres per cell |
0 | 0d | 0 | 0 | N/Ae | 0 |
| No. of cells analyzed | 3 | 116 | 3 | 3 | N/A | 17 |
| Ab10/N10 Mean no. of neocentromeres per cell ±sd |
2.2 ± 1.2 | 2.0 ± 1.4 | 0.6 ± 0.5 | 1.4 ± 1.2 | N/A | 2.6 ± 1.6f |
| No. of cells analyzed | 6 | 23 | 15 | 53 | N/A | 64 |
| Percent of cells with neocentromeres | 100 | 83 | 40 | 72 | N/A | 88 |
a See Methods for a description of treatments.
b Plants were full siblings, varying by the presence of the Ab10 chromosome.
c AI, anaphase I; AII, anaphase II.
d A single neocentromere (or what appeared to be one) was detected in 116 cells.
e N/A, data not available.
f
At anaphase II, the number of neocentromeres in the taxol treatment was significantly different from the number in the cultured treatment (P < 0.01) but not the fixed treatment.