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. 2003 Apr 15;111(8):1181–1190. doi: 10.1172/JCI16651

Figure 7.

Figure 7

Binding of recombinant mutant, mutant factor H (E1172Stop), and wild-type proteins to endothelial cells. (a) Dose-dependent binding of recombinant wild-type factor H (FH 8-20). HUVECs incubated in serum-free medium were incubated with the indicated amounts of purified recombinant wild-type factor H 8-20, and binding was assayed by FACS analysis. (b) Comparison of recombinant mutant R1210C (i.e., FH 8-20/R1210C), or R1215G (i.e., FH 8-20/R1215G), or wild-type factor H (FH 8-20). HUVECs cultivated in serum-free medium were incubated with the indicated proteins, and binding was assayed by FACS analysis. (c) Binding of purified factor H protein with the E1172Stop mutation isolated from serum of patient R043 and of purified wild-type factor H to HUVECs. HUVECs were treated with either 5 μg of mutant factor H protein purified from plasma of patient R043 or with 5 μg purified intact wild-type factor H, and binding was assayed by FACS.