Figure 3.

Neuronal differentiation in the ischemic striatum. (a and c) Representative confocal microscopic images of BrdU, DCX, and NeuN colabeling showing that BrdU-labeled neurons (arrowheads) were located in the boundary zone of the ischemic lesion at 5 (a) and 13 (c) weeks. A white solid line delineates the lesion area. Scale bar is 50 μm. (b and d) Confocal 3D analyses of BrdU⁺DCX⁺ cells at 5 weeks (b) and BrdU⁺NeuN⁺ cells at 13 weeks (d) after ischemia. Reconstructed orthogonal images are presented as viewed from the sides in both the x-z (top) and y-z (right) planes. Arrowheads indicate double-labeled cells. Scale bar is 10 μm. n = 5–6 per group (for quantification, see Table 1).