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(A) UV-vis spectra of reduced CuBMb in air (solid line) and that in O2 saturated buffer (dotted line). (Inset) High-spin ferrous WTMb (dotted line) and CuBMb (solid line). (B) Reduced CuBMb in air (solid line), reduced CuBMb in air after the addition of AgNO3 (dashed line), and reduced WTMb in air (dotted line). Spectra were recorded in 20 mM Tris buffer (pH 8) at 25°C.
