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. 2003 Mar 24;100(7):3677–3682. doi: 10.1073/pnas.0737682100

Figure 2.

Figure 2

The pooled iron siderophore fraction of E. coli CA46 (a–c) eluted from DE52-cellulose with 2 M ammonium chloride was adjusted to pH 2 with H2SO4, extracted with ethyl acetate, and analyzed by HPLC. (a) The ethyl acetate extract contained the linear degradation products monomer DHBS (M), the dimer (DHBS)2 (D), and the trimer (DHBS)3 (T), and traces of the cyclic enterochelin (E) were identified. In the water phase of the ethyl acetate extract (b), the monomer, aerobactin (A), and salmochelins 1–3 (S1–S3) were found. (c) The ammonium chloride eluate of the DE52-cellulose column contained mainly salmochelins 1–3 and aerobactin. (d) The mutant Salmonella H5546 iroBC∷kan produced only enterochelin and the linear degradation products. (e) The mutant Salmonella H5547 iroD∷kan secreted the monomer and S4, which represents an unidentified compound that awaits further characterization.