Table 3.
Growth promotion of S. enterica serotype Stanleyville strains by salmochelin
| S. enterica serotype Stanleyville strain | Relevant mutation | Growth zone diameter, mm |
|---|---|---|
| 21 | ||
| WR1361 | cir | 21 |
| WR1359 | iroN | 18 |
| WR1366 | iroN fepA | (17) |
| WR1367 | cir fepA | 20 |
| WR1363 | iroN cir | 0 |
| WR1368 | iroN cir fepA | 0 |
Nutrient broth plates containing dipyridyl to limit available iron were seeded with the indicated strains suspended in 2.5 ml of soft agar in water. Filter-paper discs impregnated with 15 μl of deferri-salmochelin solution (≈1 mg/ml) were applied, and growth zones were measured after 18 h. Numbers in bold indicate good growth; normal numbers and numbers in parentheses indicate poor and very poor growth, respectively.