Table 2.

Metal content of DtxR as isolated and after reconstitution

Metal	Metal/monomer in DtxR
	Wild type	H79A	M10A
As isolated
Fe	0.012 ± 0.006 (n = 7)	0.009 ± 0.005 (n = 3)	0.042 ± 0.003 (n = 2)
Ni	0.09  ± 0.03 (n = 7)	0.003 ± 0.001 (n = 3)	0.071 ± 0.004 (n = 2)
Zn	0.07  ± 0.03 (n = 7)	0.02  ± 0.01 (n = 3)	0.044 ± 0.003 (n = 2)
After reconstitution
Mn2+	1.3   ± 0.2 (n = 3)	0.0009 (n = 3)	0.21  ± 0.02 (n = 2)
Co2+	1.6   ± 0.2 (n = 4)	0.35  ± 0.08 (n = 3)	0.73  ± 0.03 (n = 2)
Fe2+	2.0   ± 0.3 (n = 6)	0.6   ± 0.1 (n = 3)	0.81  ± 0.03 (n = 2)
Ni2+	2.5   ± 0.7 (n = 5)	0.55  ± 0.09 (n = 3)	1.22  ± 0.04 (n = 2)
Fe3+	22    ± 7 (n = 5)	33    ± 3 (n = 3)	23    ± 5 (n = 2)

The DtxR concentration was determined for each sample. All concentrations were in the 10 μM range.