Table 1.
Plasmids | Primers |
---|---|
pRSSC644A | CCG ATT GAT GAG GCC TAC GAG |
CTC GTA GGC CTC ATC AAT CGG | |
pRSSC647A | C TAC GAG GCT GGC TTT AC |
GT AAA GCC AGC CTC GTA G | |
pRSSC662A | C TTC ACT GCC CCG AAA TG |
CA TTT CGG GGC AGT GAA G | |
pRSSC665A | CCG AAA GCT GGT AAC CAT GAC |
GTC ATG GTT ACC AGC TTT CGG |
Plasmids encoding mutated protein α and corresponding primers for site-directed mutagenesis. The primers (upper) and their complements (lower) are broken into codons, and the changed bases are in bold.