Figure 4.

Electrophoretic resolution of 5HT and 5HTrp photoproducts in 10 μs. Here, a field estimated to be ≈0.15 MV⋅cm⁻¹ (35 kV) was used to fractionate components over a separation distance of 9 μm. The sample solution contained deoxygenated phosphate buffer (5 mM, pH 7.0) and 5HT and 5HTrp (both at 500 μM). Approximately 65,500 repeat electrophoretic events were summed to generate this trace, and the data-bin size was 640 ns. The nominal gate duration was 1 μs, and the probe beam was operated at ≈100 mW.