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. 2003 Mar 12;100(7):3989–3994. doi: 10.1073/pnas.0736565100

Figure 1.

Figure 1

Association of endogenous cadherins with CDO and BOC. (A and B) Association of CDO, cadherin, and β-catenin in C2C12 cells. Lysates from proliferating, low-density C2C12 cells cultured in growth medium (G) or confluent cultures in differentiation medium [2% horse serum (HS)] for the indicated times were immunoprecipitated with antibodies to CDO (A) or cadherin (pancadherin antibody) (B), fractionated by SDS/PAGE, and immunoblotted with the indicated antibodies. Straight lysates were also probed as indicated. Immunoblotting for myogenin and MHC indicates the progression of cell differentiation. (C) Association of N- and M-cadherin with CDO. (D) Association of BOC, cadherin, and β-catenin in RD cells. (E) Association of cadherin and β-catenin with stably expressed BOC(flag) in C2C12 cells. (F) Association of N- and M-cadherin with stably expressed BOC(flag) in C2C12 cells. For C–F, immunoprecipitation and immunoblotting are as described for A and B.