Figure 6.

Concentration of PI4KIIα at synapses and in the Golgi complex area. Rat brainstem frozen sections were stained by double immunofluorescence. (A and B) PI4KIIα colocalizes with synaptojanin 1 and synaptobrevin 2/VAMP2 at synapses, which appear as bright fluorescence puncta outlining perikarya and dendrites. (C) Bright perinuclear PI4KIIα immunofluorescence is located in close apposition (see Insets) to elements immunoreactive for the cis-Golgi complex marker GM130. Small arrows point to synapses. In the “merged” images, PI4KIIα immunoreactivity appears in red. (Bar = 30 μm in A and 10 μm in B and C.)