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. 2003 Mar 19;100(7):4072–4077. doi: 10.1073/pnas.2628038100

Figure 1.

Figure 1

N-terminal (A) and C-terminal (B) truncations of the 27-residue AKB domain of D-AKAP2. Truncated peptides were synthesized by using SPOT synthesis on cellulose membrane as described in Materials and Methods. Binding was evaluated by incubating each membrane with GFP-RIα D/D domain and GFP-RIIα D/D domain as indicated. Bound protein was detected by using a primary antibody against GFP and enzyme-conjugated secondary antibody for amplification of signal. The membrane was then analyzed by chemiluminescence.