Figure 1.
N-terminal (A) and C-terminal (B) truncations of the 27-residue AKB domain of D-AKAP2. Truncated peptides were synthesized by using SPOT synthesis on cellulose membrane as described in Materials and Methods. Binding was evaluated by incubating each membrane with GFP-RIα D/D domain and GFP-RIIα D/D domain as indicated. Bound protein was detected by using a primary antibody against GFP and enzyme-conjugated secondary antibody for amplification of signal. The membrane was then analyzed by chemiluminescence.