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Binding of AKB(dual) (■), AKB(RI) (Q9F, V21W, M25F) (⧫), AKB(RII) (A13L) (▴), and AKB(null) (●) peptides to full-length RIα (A) and RIIα (B). Each peptide was fluorescently labeled and incubated with the corresponding regulatory subunit for 1 h in 10 mM Hepes, 150 mM NaCl, 3 mM EDTA, pH 7.4. Fluorescence anisotropy was used to monitor bound peptide.
