Figure 5.

Transcriptional activity of a series of deletion constructs of TNF-α promoter DNA. (a) The activity was measured due to the promoter wtTNFP (−991 to 1) or mtTNFP1 (−991 to 1Δ−515 to −511). pGL3-basic transfected cell was used as unstimulated control. After transfection of DNAs for 3 h, the cells were washed with PBS twice and stimulated with 100 ng/ml LPS (E. coli) or transfected with Chariot/peptide complex of 1 μg/ml peptide A, B, C, or HA. Triplicate assays were performed. Values are normalized by β-galactosidase production and graphed. (b) The activity was measured due to the promoter mtTNFP2 (−550 to −487 plus TATA box) or mtTNFP3 (−550 to −487Δ−515 to −511 plus TATA box). LPS or peptides as stimuli and controls were used at the same condition as described above.